The Furman Lab The Hebrew University of Jerusalem Department of Microbiology and Molecular Genetics, Faculty of Medicine, Hadassah Medical School Institute for Medical Research Israel-Canada (IMRIC)

Welcome!

Our laboratory consists of interacting scientists that are interested in improving our basic understanding and manipulation of interactions between proteins, using a combination of computational and experimental approaches.

This embraces different levels of resolution and scale: starting from the basic atom - level details of interactions; continuing to prediction and characterization of specific interactions; and finally addressing the ultimate question of their role within the context of a cell and a whole organism.

Our computational tools include the structure-based computational prediction and manipulation of specific interactions using the Rosetta modeling framework, analysis of evolutionary signals hidden in sequences, and large-scale integration of this data by machine-learning approaches.

Our ERC starting grant has made it possible for us to realize a dream and to open a wet lab. Our experimental setup includes the characterization of interactions using biophysical methods such as ITC, as well as in vitro selection with Yeast surface Display. In addition, we have embarked on the journey of characterizing the functional role of proteins with repeated domains for the adaptability of yeast to environmental changes. Read More »

Positions available for PhD Students and PostDocs

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News

(9/2017) PIPER-FlexPepDock is now online!

PIPER-FlexPepDock is a fragment-based protocol for high-resolution global peptide-protein docking.

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Selected Publications

Check out our full publication list

Derived Peptides

• London N, Raveh B, Movshovitz-Attias D, Schueler-Furman O (2010).
  Can self-inhibitory peptides be derived from the interfaces of globular protein-protein interactions? Proteins 78(15):3140-9.
• Sedan, Marcu, Lyskov & Schueler-Furman (2016).
  Peptiderive server: derive peptide inhibitors from protein-protein interactions. Nucleic Acids Res. 44(W1):W536-541.

Peptide-protein interactions within their context

• Dodson EJ, Fishbain-Yoskovitz V, Rotem-Bamberger S, Schueler-Furman O (2015).
  Versatile communication strategies among tandem WW domain repeats. Exp Biol Med 240:351-360.

Comprehensive Reviews on peptide-peptide docking and the peptide-based inhibition of protein-protein interactions

• London, Raveh, Schueler-Furman (2013)
  Peptide docking and structure-based characterization of peptide binding: from knowledge to know-how. Curr Opin Struct Biol 23:894-902.
• London, Raveh, Schueler-Furman (2013)
  Druggable protein-protein interactions-from hot spots to hot segments. Curr Opin Chem Biol 17:952-959.

Protein-protein association energy landscapes

• Kozakov, Li, Hall, Beglov, Zheng, Vakili, Schueler-Furman, Paschalidis, Clore, Vajda (2014)
  Encounter complexes and dimensionality reduction in protein-protein association. Elife 3:e01370.

Binding Specificity & Design

• Slutzki, Reshef, Barak, Haimovitz, Rotem-Bamberger, Lamed, Bayer, Schueler-Furman (2015).
  Crucial roles of single residues in binding affinity, specificity and promiscuity in the cellulosomal cohesin-dockerin interface. JBC in press.
• Gao M, London N, Cheng K, Tamura R, Jin J, Schueler-Furman O, Yin H. (2014).
  Rationally Designed Macrocyclic Peptides as Synergistic Agonists of LPS-Induced Inflammatory Response. Tetrahedron 70:7664-7668.
• London, Lamphear, Hougland, Fierke & Schueler-Furman (2011).
  Identification of a novel class of farnesylation targets by structure-based modeling of binding specificity. PLoS Comput Biol 7:e1002170.
• Al-Quadan, Price, London, Schueler-Furman & Abukwaik (2011).
  Anchoring of bacterial effectors to host membranes through host-mediated lipidation by prenylation: a common paradigm. Trends Microbiol. 19:573-579.

FlexPepDock

• London, Raveh, Cohen, Fathi & Schueler-Furman (2011).
  Rosetta FlexPepDock web server--high resolution modeling of peptide-protein interactions.Nucleic Acids Res 39(Web Server issue):W249-53.
  
• Raveh, London, Zimmerman & Schueler-Furman (2011).
  Rosetta FlexPepDock ab-initio: Simultaneous Folding, Docking and Refinement of Peptides onto Their Receptors. PLoS One 6:e18934.
• Belitsky, Avshalom, Erental, Yelin, Kumar, London, Sperber, Schueler-Furman & Engelberg-Kulka (2011).
  The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBK. Mol Cell 41:625-35.